Multiplexed assay and probes for identification of HPV types

US7875428B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Nader Pourmand · San Mateo, US
• Baback Gharizadeh · Menlo Park, US
• Ronald W. Davis · Menlo Park, US

Key dates

• Filing date: Feb 13, 2007
• Grant date: Jan 25, 2011
• Expiry date: Dec 14, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/708
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A DNA microarray, preferably in the form of a chip, contains probes which hybridize to generate primers capable of amplifying approximately 89 HPV types. These target the E1 region of the gene. The design of the chip allows for the detection of any known HPV type, based on a unique probe sequence derived from the HPV E1 region. The present assay utilizes a number of primers that can amplify from about one to six different types of HPV. A large number of primers can be used together. After amplification, the amplicons are contacted with specific probes that are unique for each HPV type. The array further employs a control sequence, which normalizes variability due to sample size.