Non-desensitizing mutant of the transient receptor potential TRPM5 ion channel

US7875452B2 · kind B2 · utility

Assignee

• Redpoint Bio Corporation · US

Inventors

• Robert W. Bryant · Princeton, US
• S. Paul Lee · Phoenix, US
• Rok Cerne · Lawrenceville, US
• M. N. Tulu Buber · Newtown, US
• Ivona Bakaj · Cranbury, US
• Roy Kyle Palmer · Cranbury, US

Key dates

• Filing date: Sep 30, 2008
• Grant date: Jan 25, 2011
• Expiry date: Sep 30, 2028

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods, especially electrophysiological, suffer from a lack of sensitivity, rapid signal loss, low throughput, and are labor intensive. The claimed methods and compositions provide electrophysiology methods that allow prolonged sample testing and fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.