Method of analyzing DNA sequence using field-effect device, and base sequence analyzer
US7888013B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 26, 2005 |
| Grant date | Feb 15, 2011 |
| Priority date | — |
| Expiry date | Jun 27, 2027 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0877
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Since conventional DNA sequence analyzing technologies are based on the fundamental principle of fluorescent detection, expensive, complex optical systems and laser sources have been necessary.A field-effect device for gene detection of the present invention analyzes a base sequence by immobilizing a single-strand nucleic acid probe at a gate portion, inducing hybridization at the gate portion to form a double-stranded DNA, inducing elongation reaction by adding a DNA polymerase and one of the substrates, and measuring the electrical characteristic of the field-effect device caused by elongation reaction.Since the elongation reaction of one base induced at the gate portion can be directly converted to an electrical signal, expensive lasers or complex optical systems are not needed. Thus, a small gene polymorphism detection system that can conduct measurement at high precision can be provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.