Method of performing PCR amplification on a microarray
US7892732B2 · kind B2 · utility
1Cited by
3References
2Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 12, 2005 |
| Grant date | Feb 22, 2011 |
| Priority date | — |
| Expiry date | May 3, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of amplifying target DNA by PCR or multiplex PCR on a microarray using array-immobilized DNA probes synthesized in a common area on the microarray by a maskless array synthesizer (MAS). The MAS constructed array-immobilized DNA probes include a universal primer linked to a sequence-specific probe, and optionally a calibrated probe for use in quantifying amplified target DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.