Enhanced sensitivity of a cantilever sensor via specific bindings
US7892759B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 15, 2008 |
| Grant date | Feb 22, 2011 |
| Priority date | — |
| Expiry date | Sep 20, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54373
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Detection of miniscule amounts of an analyte is accomplished via multiple bindings of specific materials on a sensor configured to sense mass. The sensor is prepared by immobilizing an antibody to a surface of the sensor, wherein the antibody is known to bind to the analyte. The prepared sensor is exposed to the analyte. The analyte binds to the antibody. The sensor then is exposed to additional antibody, which binds to the analyte. The sensor then can be sequentially exposed to additional antibodies that are known to bind to previously bound antibodies. Each additional binding further increases the effective mass of accumulated material on the sensor. The total effective mass is greater than the mass of the accumulated analyte, thus providing means for detecting extremely minute amounts of analyte. Applications include detection of pathogens and DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.