Single enzyme system for fast, ultra long PCR
US7892797B2 · kind B2 · utility
10Cited by
8References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | May 5, 2008 |
| Grant date | Feb 22, 2011 |
| Priority date | — |
| Expiry date | May 5, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods, formulation and kits for the synthesis of long nucleic acid fragments. An improved PCR method is provided for amplifying long DNA fragments. In particular, a single thermostable DNA polymerase is used for the rapid amplification of over 10 kb long DNA fragments. Also provided is a method for extending long complementary DNA strands using this single enzyme formulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.