Clonal culture of human pluripotent stem cells
US7892830B2 · kind B2 · utility
12Cited by
8References
8Claims
0Family size
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Key dates
| Filing date | Jan 17, 2008 |
| Grant date | Feb 22, 2011 |
| Priority date | — |
| Expiry date | Jan 17, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/999
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
While culture medium and systems have been described that permit the culture and proliferation of human embryonic stem cells in feeder free and animal product free conditions, these conditions will not readily support cloning of an embryonic stem cell culture meaning, at least here, the initiation of a sub-culture using one or a very few originating cells. It has been found here that a class of small molecules that are inhibitors of kinase enzymes will increase the efficiency of cloning of stem cell cultures sufficiently to make such cloning practical in the defined medium and in other media as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.