Patent · US Active

Assay by osmotically induced separation and concentration of high-molecular detectable substances and a fluid microsystem for carrying out said assay

US7892857B2 · kind B2 · utility

0Cited by

3References

10Claims

0Family size

Inventors

Rudolf Ehwald · Berlin, DE
Helge Adleff · Berlin, DE
Frank Bier · Potsdam, DE
Nenad Gajovic-Eichelmann · Berlin, DE
Max Ehwald · Berlin, DE

Key dates

Filing date	Aug 30, 2007
Grant date	Feb 22, 2011
Priority date	—
Expiry date	Nov 5, 2028

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/968
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to an assay method and a fluid microsystem for carrying out said assay method, in particular, for carrying out miniaturized affinity tests in a micro-array format. According to said invention, a liquid phase comprising at least one type of detectable high-molecular weight soluble substance, for example a labeled reaction partner or product of an affinity reaction is displaced by the hydraulic effect of a high-molecular weight osmotic agent on an ultrafiltration membrane towards a miniaturized measuring chamber defined thereby. The fraction of the detectable high-molecular weight substance(s) is concentrated in the measuring chamber, while the dissolved low-molecular weight components are removed with the solvent through the membrane pores, thereby making it possible to attain a high detection sensitivity.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.