Patent · US Expired

Stabilisation of double-stranded nucleic acids using proteins

US7893016B2 · kind B2 · utility

1Cited by

2References

11Claims

0Family size

Assignee

GE Healthcare UK Limited · GB

Inventors

Navin Deepal Pathirana · Cape Elizabeth, US
Neil Butt · Cambridge, GB
Matthew Baker · Maidstone, GB

Key dates

Filing date	Dec 11, 2002
Grant date	Feb 22, 2011
Priority date	—
Expiry date	Jun 21, 2024

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/1003
WIPO fieldOrganic fine chemistry
WIPO sectorChemistry

Abstract

A composition for treating a nucleic acid duplex, wherein the composition is capable of inhibiting denaturation of the duplex, characterised in that the composition comprises a ubiquitin-like protein and/or a macroglobulin. It has been found that ubiquitin-like proteins (e.g. ubiquitin, NEDD8, RAD23, etc.) and macroglobulins (eg. α2-macroglobulin) are able to stabilise nucleic acid duplexes. A nucleic acid duplex which has been contacted with the composition of the invention can be subjected to more stringent processing conditions, with denaturation of the duplex being inhibited, than would otherwise be possible. Corresponding methods and uses are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.