Patent · US Expired

Process for renaturation of recombinant, disulfide containing proteins at high protein concentrations in the presence of amines

US7893210B2 · kind B2 · utility

1Cited by

7References

5Claims

0Family size

Assignee

Bayer Schering Pharma AG · DE

Inventors

Jorg Peters · Düsseldorf, DE
Torsten Minuth · Wuppertal, DE

Key dates

Filing date	Nov 12, 2002
Grant date	Feb 22, 2011
Priority date	—
Expiry date	May 25, 2025

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K14/8117
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for renaturation of proteins comprising adding to a solution of denatured, chemically modified or reduced proteins a refolding buffer containing a primary, secondary or tertiary amine. Said method has been applied, for example, to interleukin-4 and bovine pancreatic trypsin inhibitor (BPTI), which were previously (i) solubilized in the presence of guanidinium hydrochloride as chaotronic agent, and (ii) subjected to sulfitolysis.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.