Methods for selective isolation of nucleic acids from microbial cells present in samples containing higher eukaryotic cells and/or tissues
US7893251B2 · kind B2 · utility
18Cited by
2References
21Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 28, 2006 |
| Grant date | Feb 22, 2011 |
| Priority date | — |
| Expiry date | Mar 16, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to the use of nucleases, especially DNA-degrading nucleases, for degrading nucleic acids in the presence of one or several chaotropic agents and/or one or several surfactants. The invention further relates to a method for purifying RNA from mixtures of DNA and RNA as well as kits for carrying out such a method. Also disclosed is a method for specifically isolating nucleic acids from microbial cells provided in a mixed sample which additionally comprises higher eukaryotic cells as well as kits for carrying out such a method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.