Conjugated polymers suitable for strand-specific polynucleotide detection in homogeneous and solid state assays

US7897684B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Guillermo C. Bazan · Lompoc, US
• Bin Liu · Fishers, US

Key dates

• Filing date: Jan 10, 2006
• Grant date: Mar 1, 2011
• Expiry date: Sep 24, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6439
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention further relates to polycationic multichromophores, which may be conjugated polymers, and methods, articles and compositions employing them as described herein. In some aspects, the invention relates to methods, articles and compositions for the detection and analysis of biomolecules in a sample. Provided assays include those determining the presence of a target biomolecule in a sample or its relative amount, or the assays may be quantitative or semi-quantitative. The methods can be performed on a substrate. The methods can be performed in an array format on a substrate, which can be a sensor. In some embodiments, detection assays are provided employing sensor biomolecules that do not comprise a fluorophore that can exchange energy with the cationic multichromophore. In some aspects biological assays are provided in which energy is transferred between one or more of the multichromophore, a label on the target biomolecule, a label on the sensor biomolecule, and/or a fluorescent dye specific for a polynucleotide, in all permutations. The multichromophore may interact at least in part electrostatically with the sensor and/or the target, and an increase in energy transfer …