Methods for identifying sets of oligonucleotides for use in an in vitro recombination procedures

US7904249B2 · kind B2 · utility

Assignee

• Codexis Mayflower Holdings, Inc. · US

Inventors

• Sergey Selifonov · Plymouth, US
• Willem P. C. Stemmer · Los Gatos, US
• Claes Gustafsson · Belmont, US
• Matthew Tobin · San Jose, US
• Stephen del Cardayre · South San Francisco, US
• Phillip A. Patten · Portola Valley, US
• Jeremy Minshull · Los Altos, US
• Lorraine Giver · Sunnyvale, US

Key dates

• Filing date: Oct 31, 2007
• Grant date: Mar 8, 2011
• Expiry date: Jul 9, 2028

Classification

• Technology area (CPC G): Physics
• CPC primary: G16B40/00
• WIPO field: Computer technology
• WIPO sector: Electrical engineering

Abstract

In silico nucleic acid recombination methods, related integrated systems utilizing genetic operators are used to identify sets of oligonucleotides for in vitro recombination. In certain embodiments, the methods involve inputting amino acid sequence character strings into a digital system and back-translating the amino acid character strings into a plurality of nucleic acid character strings. The nucleic acid character strings are then processed to define and select at least one recombinant nucleic acid for use in identifying one or more oligonucleotides for in vitro recombination.