Compare-MS: method rapid, sensitive and accurate detection of DNA methylation
US7906288B2 · kind B2 · utility
2Cited by
8References
43Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 4, 2007 |
| Grant date | Mar 15, 2011 |
| Priority date | — |
| Expiry date | Jan 4, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/683
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods and kits useful for enriching, identifying and quantifying methylated DNA3 particularly hypermethylated CpG islands by digesting a sample with a methylation-sensitive restriction endonuclease and capturing methylated restriction fragments with a methyl-binding capture reagent. The methods of the invention may be used in the detection of cancer, particularly detection of prostate cancer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.