Thermostable Photinus pyralis luciferase mutant

US7906298B1 · kind B1 · utility

Assignee

• Promega Corporation · US

Inventors

• David James Squirrell · Salisbury, GB
• Melenie Jane Murphy · Salisbury, GB
• Rachel Louise Price · Devizes, GB
• Christopher Robin Lowe
• Peter John White · Cambridge, GB
• Laurence Carlo Tisi · Cambridge, GB
• James A. Murray · Walnut Creek, US

Key dates

• Filing date: Oct 26, 1999
• Grant date: Mar 15, 2011
• Expiry date: Oct 26, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/66
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A protein having luciferase activity and at least 60% similarity to luciferase from Photinus pyralis, Luciola mingrelica, Luciola cruciata or Luciola lateralis. Hotaria paroula, Pyrophorus plagiophtalamus, Lampyris noctiluca, Pyrocoelia nayako or Photinus pennsylanvanica wherein in the sequence of the enzyme, at least one of (a) the amino acid residue corresponding to residue 214 in Photinus pyralis luciferase; (b) the amino acid residue corresponding to residue 232 in Photinus pyralis luciferase; (c) the amino acid residue corresponding to residue 295 in Photinus pyralis luciferase; (d) the amino acid residue corresponding to acid 14 of Photinus pyralis luciferase; (e) the amino acid residue corresponding to amino acid 35 of Photinus pyralis luciferase; (f) the amino acid residue corresponding to amino acid residue 105 of Photinus pyralis luciferase; (g) the amino acid residue corresponding to amino acid residue 234 of Photinus pyralis luciferase; (h) the amino acid residue corresponding to amino acid residue 420 of Photinus pyralis luciferase; (i) the amino acid residue corresponding to amino acid residue 310 of Photinus pyralis luciferase; is different to the amino acid which ap…