Reaction sensing in living cells
US7910065B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 16, 2009 |
| Grant date | Mar 22, 2011 |
| Priority date | — |
| Expiry date | Oct 16, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Chemical reactions occurring within a living cell are measured in a manner that does not affect the viability of the cell or the reaction under study. In one embodiment, one or more sensors are introduced into the cell and/or covalently associated with the exterior cell membrane. The sensor(s) emit an observable signal indicating a value of a parameter associated with the chemical reaction, e.g., the concentration of a reaction product. Because cell viability is not compromised, the cell may be stimulated (e.g., by subjection to an agonist or antagonist, a pathogen, a pharmaceutical compound, or a potential toxin) so as to affect the reaction under study.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.