Patent · US Active

Method for manufacturing a recombinant polyclonal protein

US7910332B2 · kind B2 · utility

6Cited by
2References
59Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 23, 2008
Grant dateMar 22, 2011
Priority date
Expiry dateSep 3, 2028

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC07K16/081
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention relates to a method for manufacturing a recombinant polyclonal protein composition, in particular a recombinant polyclonal antibody composition. The method comprises obtaining a collection of cells transfected with a collection of variant nucleic acid sequences, wherein each cell in the collection is transfected with and capable of expressing one member of the collection, which encodes a distinct member of a polyclonal protein. The cells are cultured under suitable conditions for expression of the polyclonal protein, which is obtained from the cells or culture supernatant. The nucleic acid sequence is introduced into the cells by transfection with a collection of vectors. The present method is suitable for manufacturing recombinant polyclonal antibodies for therapeutic uses.

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