Methods and compositions for use in analyte detection using proximity probes
US7914987B2 · kind B2 · utility
9Cited by
4References
33Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 14, 2005 |
| Grant date | Mar 29, 2011 |
| Priority date | — |
| Expiry date | Aug 18, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and compositions for detecting an analyte in a sample are provided. In practicing the subject methods, a sample is combined with at least a pair of proximity probes that each include an analyte binding domain and a nucleic acid domain. The resultant mixture is then contacted with a pair of asymmetric nucleic acid connectors. Proximity dependent connector mediated interaction between the nucleic acid domains of the proximity probes is then detected to determine the presence of the analyte in the sample. Also provided are kits and systems for practicing the subject methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.