Capture moieties for nucleic acids and uses thereof

US7914989B2 · kind B2 · utility

Assignee

• Luminex Molecular Diagnostics, Inc. · CA

Inventors

• Susan Bortolin · Oakville, CA
• Roman Zastawny · Mississauga, CA

Key dates

• Filing date: May 25, 2007
• Grant date: Mar 29, 2011
• Expiry date: Dec 4, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6818
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A unimolecular probe for hybridization to a molecule comprising a target nucleic acid sequence, the probe includes: a first nucleic acid sequence complementary to the target sequence (target-binding sequence); and a second nucleic acid sequence complementary to a portion of the first nucleic acid sequence and capable of hybridization therewith to form a first intramolecular duplex. In use, the target and target-bind sequence hybridize to form a duplex. A probe can be used to detect a molecule containing the target sequence, act as a primer for synthesis or amplification, etc.