Method for separating an analyte from a sample

US7914994B2 · kind B2 · utility

Assignee

• Cepheid · US

Inventors

• Kurt E. Petersen · San Jose, US
• William A. McMillan · Cupertino, US
• Lee A. Christel · Palo Alto, US
• Ronald Chang · Redwood City, US
• Farzad Pourahmadi · Fremont, US
• Jesus Ching · Saratoga, US
• Gregory T. Kovacs · Palo Alto, US
• M. Allen Northrup · Berkeley, US

Key dates

• Filing date: Feb 12, 2009
• Grant date: Mar 29, 2011
• Expiry date: Feb 12, 2029

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/255
• WIPO field: Chemical engineering
• WIPO sector: Chemistry

Abstract

An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path.