Host cell modifications that improve peptide production and downstream processing
US7915011B2 · kind B2 · utility
1Cited by
10References
22Claims
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Key dates
| Filing date | Oct 8, 2009 |
| Grant date | Mar 29, 2011 |
| Priority date | — |
| Expiry date | Oct 8, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disrupting the expression of endogenous Escherichia host cell genes gcvA and spr provides mutant host cells having increased heterologous peptide production. The addition of a genetic modification to the coding region of gene yejM further enhances peptide production and facilitates easier downstream processing. Recombinant Escherichia host cells are provided as well as methods of using such host cells for heterologous peptide production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.