Method of amplifying nucleic acid from a cell using a nonplanar solid substrate
US7919278B2 · kind B2 · utility
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12Claims
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Key dates
| Filing date | Aug 20, 2007 |
| Grant date | Apr 5, 2011 |
| Priority date | — |
| Expiry date | Aug 30, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.