In vitro method for the diagnosis of cardiovascular functionality of bone marrow precursor cells (BMPS) and/or blood-derived circulating precursor cells (BDPS)
US7919315B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 13, 2004 |
| Grant date | Apr 5, 2011 |
| Priority date | — |
| Expiry date | May 11, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/755
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to an in vitro method for the analysis of a sample from a mammal in connection with cardiovascular diseases, wherein the method comprises the following steps: a) isolating of bone marrow-precursor cells (BMPs) and/or blood-derived circulating precursor cells (BDPs) by means of cell specific surface markers, and b) detecting the cardiovascular functionality of the isolated BMPs and/or BDPs by means of a suitable migration assay. The method according to the invention can be employed as a kit in the context of the diagnosis and/or the prognosis of cardiovascular diseases, for the monitoring of their therapies and/or for a stratification for a prospective cell therapy with stem- and/or precursor cells in order to increase the perfusion of ischemic tissue or for a regeneration of tissue losses (e.g. heart insufficiency), respectively. In a further aspect, the present invention then relates to an in vitro method for isolating specific bone marrow-precursor cells by means of a suitable migration assay. According to the invention, these BMPs and/or BDPs can be used for the treatment of cardiovascular diseases, selected from the group consisting of stable coron…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.