Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells
US7919318B2 · kind B2 · utility
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Key dates
| Filing date | Jun 13, 2007 |
| Grant date | Apr 5, 2011 |
| Priority date | — |
| Expiry date | Jun 13, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2509/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
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