Perhydrolase providing improved peracid stability

US7923233B1 · kind B1 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Robert DiCosimo · Chadds Ford, US
• John Edward Gavagan · Wilmington, US
• Mark S. Payne · Wilmington, US

Key dates

• Filing date: Dec 7, 2009
• Grant date: Apr 12, 2011
• Expiry date: Dec 7, 2029

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61L2202/26
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in the ratio of peracetic acid formation to peracetic acid hydrolysis specific activities (PAAF/PAAH ratio). The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.