Method of purifying RNA using kosmotropic salt
US7923551B2 · kind B2 · utility
3Cited by
1References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 28, 2008 |
| Grant date | Apr 12, 2011 |
| Priority date | — |
| Expiry date | Apr 17, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1006
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.