Patent · US Active

Injection method for microfluidic chips

US7927476B2 · kind B2 · utility

8Cited by
9References
10Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 22, 2008
Grant dateApr 19, 2011
Priority date
Expiry dateAug 6, 2029

Classification

  • Technology area (CPC B)Performing Operations; Transporting
  • CPC primaryB01D57/02
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

Methods and systems for injecting a sample during electrophoresis, that generally comprise: loading a sieving matrix through a first end of a separation channel; having the an end of the sieving matrix at a set distance from the intersection of the separation channel and a loading channel; pressure loading a sample through the loading channel and filling an empty portion of the separation channel; applying an electric field across the separation channel while flowing a washing buffer through the loading channel; and injecting a portion of the sample into the separation channel, wherein the portion of the sample injected is of a size that is determined by a distance between the end of the sieving matrix and the intersection of the loading and separation channels.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.