Rapid particle detection assay
US7927561B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 15, 2008 |
| Grant date | Apr 19, 2011 |
| Priority date | — |
| Expiry date | Apr 28, 2029 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/043
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention proves instruments and methods for detecting and/or quantitating an analyte in a fluid sample. The fluid sample is placed in a sample chamber having a small, shallow detection region. The analyte is magnetically labeled using magnetic particles coated with a binding reagent, and is detectably labeled using a fluorescent dye or other detection reagent. The magnetically labeled analyte is concentrated into the detection region using a focusing magnet positioned underneath the sample chamber detection region. Concentrated analyte is measured using excitation optics positioned on top of the sample chamber detection region, adapted to illuminate only the detection region, and detection optics positioned on top of the detection region, adapted to detect only light emitted from the detection region. In a preferred embodiment, the invention provides a simple, rapid assay for measuring the concentration of CD4+T cells in a whole blood sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.