Methods for identifying a biosynthetic pathway gene product

US7927794B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Jay D. Keasling · Berkeley, US
• Jack Newman · San Francisco, US
• Douglas J. Pitera · Oakland, US
• Sydnor T. Withers, III · Madison, US
• Keith Kinkead Reiling · Oakland, US
• Vincent Martin · Montréal, CA

Key dates

• Filing date: Sep 29, 2004
• Grant date: Apr 19, 2011
• Expiry date: Nov 12, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides isolated, genetically modified host cells, where a host cell is genetically modified with a nucleic acid that includes a nucleotide sequence encoding a biosynthetic pathway enzyme. Synthesis of the enzyme in the host cell results in conversion of a substrate for the enzyme into a biosynthetic pathway intermediate, which intermediate is produced in an amount effective to inhibit growth of the genetically modified host cell. The present invention further provides compositions and kits comprising a subject genetically modified host cell. Subject host cells are useful for identifying a gene product having activity in a biosynthetic pathway. The present invention further provides methods of identifying a gene product having activity in a biosynthetic pathway.