Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy
US7927863B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 8, 2006 |
| Grant date | Apr 19, 2011 |
| Priority date | — |
| Expiry date | May 27, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for the synthesis and secretion of recombinant proteins preferably large mammalian proteins or hetero-multimeric proteins at high levels and for prolonged time in polyploid, preferably diploid yeast. These methods use various mating competent yeast, including Pichia. In a preferred embodiment, a first expression vector is transformed into a first haploid cell; and a second expression vector is transformed into a second haploid cell. The transformed haploid cells, each individually synthesizing a non-identical polypeptide, are identified and then genetically crossed or fused. The resulting diploid strains are utilized to produce and secrete fully assembled and biologically functional hetero-multimeric protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.