Method for determining the methylation pattern of a polynucleic acid

US7932027B2 · kind B2 · utility

Assignee

• EPIGENOMICS AG · DE

Inventors

• Anne Fassbender · Berlin, DE
• Ralf Lesche · Berlin, DE
• Juergen Distler · Berlin, DE
• Christian Piepenbrock · Berlin, DE
• Tamas Rujan · Oberwil, CH
• Kurt Berlin · Stahnsdorf, DE
• Thomas Koenig · Berlin, DE

Key dates

• Filing date: Feb 16, 2006
• Grant date: Apr 26, 2011
• Expiry date: Apr 13, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6827
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Particular aspects relate to a method for determining the methylation pattern of a polynucleic acid, comprising: a) preparing a solution comprising a mixture of fragments of the polynucleic acid; b) coupling the fragments with a substance being detectable with a detection method; c) contacting a solution comprising the fragments of b) with a DNA microarray having a plurality of different immobilized oligonucleotides, each comprising at least one methylation site, at respectively assigned different locations thereon, the contacting under conditions affording hybridization of fragments with correlated immobilized oligonucleotides under defined stringency, and wherein the immobilized oligonucleotides have a length of less than 200 bases; d) optionally performing a a washing step; and e) detecting, using the physical detection method, such immobilized nucleic acids to which solution fragments are hybridized and/or to which solution fragments are not hybridized.