Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same
US7932080B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 2005 |
| Grant date | Apr 26, 2011 |
| Priority date | — |
| Expiry date | Apr 13, 2026 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An intramolecular bioluminescence resonance energy transfer (BRET), biosensor for monitoring receptor activity and signalling cascades is disclosed. The “double-brilliance” biosensor sandwiches β-arrestin (β-arr) between Renilla luciferase (Luc) and the yellow fluorescent protein (YFP). β-arr associates with G-protein coupled receptors GPCR following receptor activation, bringing Luc and YPF into close proximity that favours energy transfer. In addition to providing new insights into the agonist-induced conformational rearrangements of β-arr in living cells, the double-brilliance β-arr offers a universal biosensor for GPCR activation, allowing the study of native receptors in large-scale screening analysis. The activity of other signalling molecules known to interact with β arrestin could also be monitored by double brilliance β arr.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.