Trioxyethylene gold nanoclusters functionalized with a single DNA
US7932096B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 6, 2009 |
| Grant date | Apr 26, 2011 |
| Priority date | — |
| Expiry date | Oct 6, 2029 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00637
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of making a nanoclusters functionalized with a single DNA strand comprising the steps of providing nanoclusters, combining said nanoclusters with thiolated DNA, incubating said nanoclusters and thiolated DNA mixture, combining said mixture with a solution comprising ethanol and dichloromethane; separating said mixture into an aqueous phase and an organic phase, mixing said aqueous phase with a solution comprising dicholormethane and NaCl, and separating the mixture into an aqueous phase and an organic phase; wherein said organic phase comprises said nanoclusters functionalized with a single DNA strand. Further, provided is a nanocluster functionalized with a single DNA strand comprising a nanocluster, said nanocluster being functionalized with a single DNA strand, said DNA strand having a length of about 10 to about 50 bases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.