Method for pre-identification of spectral overlaps within fluorescent dye and detector combinations used in flow cytometry
US7932503B2 · kind B2 · utility
3Cited by
1References
38Claims
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Key dates
| Filing date | May 18, 2009 |
| Grant date | Apr 26, 2011 |
| Priority date | — |
| Expiry date | Jun 30, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6441
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to the field of flow cytometry. It provides methods for identifying important dye-detector spectral overlaps for use in designing flow cytometry experiments. It further provides methods for quantifying the impact of spectral overlaps on dye selection and detector selection, the methods including the steps of: a) obtaining spectra of a dye; b) obtaining a laser configuration and an optical filter configuration of a detector; c) obtaining a spectrum yield value; and d) ranking the spectrum yield value.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.