Multiplexed analysis for determining a serodiagnosis of viral infection

US7933721B2 · kind B2 · utility

Assignee

• The United States of America as represtented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention · US

Inventors

• Alison Johnson · Fawn Drive, AU
• Bradley John Biggerstaff · Fort Collins, US

Key dates

• Filing date: Jan 20, 2006
• Grant date: Apr 26, 2011
• Expiry date: Oct 25, 2027

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A90/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Clinical samples can be analyzed using microparticles to determine the serodiagnosis of a viral infection from two candidate viral infections of the same viral group. Serodiagnosis can be determined via a pooled population of subsets of microparticles, with the particles in the pooled population having a bound viral group-reactive antibody and the particles in each subset having at least one characteristic classification parameter that distinguishes between subsets. Viral antigens of antibodies of interest in the same viral-class as the viral group-reactive antibody can be bound to the viral group-reactive antibody on the microparticles, and subsequently exposed to a clinical sample. Binding and labeling can be used. Automated analysis of data from multiplexed flow analysis can determine the presence or absence of antibodies of interest in the sample, thereby diagnosing for two candidate viral infections in a single assay.