Polynucleotides encoding proteins for covalent tethering to functional groups and substrates
US7935803B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 10, 2008 |
| Grant date | May 3, 2011 |
| Priority date | — |
| Expiry date | May 27, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.