Laser scanning microscope
US7936503B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 11, 2008 |
| Grant date | May 3, 2011 |
| Priority date | — |
| Expiry date | Apr 25, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/0076
- WIPO fieldOptics
- WIPO sectorInstruments
Abstract
A laser scanning microscope separates fluorescence signals of different fluorophores in accurate unmixing by eliminating positional pixels shifts between different fluorescence images obtained through irradiation of different-wavelength laser lights. The microscope includes a laser light source capable of emitting a wavelength-changeable laser light, a correction amount determination unit that determines a correction amount for correcting an optical axis shift of the laser light, an optical axis adjusting unit that adjusts an optical axis, a scanning unit that performs two-dimensional scanning, an objective lens that focuses the laser scanning light to a specimen and fluorescence emitted from the specimen, a light detector that detects the fluorescence, and a control unit that changes the wavelength of the laser light synchronously with the scanning by the scanning unit while controlling the optical axis adjusting unit based on the correction amount determined by correction amount determination unit.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.