Nucleic acid amplification procedure using RNA and DNA composite primers
US7939258B2 · kind B2 · utility
18Cited by
174References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 7, 2006 |
| Grant date | May 10, 2011 |
| Priority date | — |
| Expiry date | Sep 7, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA-containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.