Method for production of recombinant human FSH
US7939296B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | May 15, 2009 |
| Grant date | May 10, 2011 |
| Priority date | — |
| Expiry date | May 15, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/59
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a method for production of recombinant human FSH in high yield and a high purity. The method comprises the steps of: (a) culturing recombinant human FSH-producing mammalian cells in a serum-free medium, (b) collecting culture supernatant, (c) subjecting the culture supernatant to cation-exchange column chromatography, (d) dye affinity column chromatography, (e) hydrophobic column chromatography, and (f) gel filtration column chromatography to collect recombinant human FSH-active fractions, in the order.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.