High speed nucleic acid sequencing

US7943305B2 · kind B2 · utility

Assignee

• Cornell Research Foundation, Inc. · US

Inventors

• Jonas Korlach · Camas, US
• Watt W. Webb · Ithaca, US
• Michael Levene · Ithaca, US
• Stephen Turner · Menlo Park, US
• Harold G. Craighead · Ithaca, US
• Mathieu Foquet · San Jose, US

Key dates

• Filing date: Apr 13, 2006
• Grant date: May 17, 2011
• Expiry date: Apr 13, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/143333
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.