Probes and primers for detection of bacterial pathogens and antibiotic resistance genes
US7943346B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 2006 |
| Grant date | May 17, 2011 |
| Priority date | — |
| Expiry date | Aug 2, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/16
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods for universal bacterial detection, for specific detection of the common bacterial pathogens, and for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. Diagnostic kits comprising such primers and probes are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.