Mutants of mycobacteria and process thereof
US7943361B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 9, 2004 |
| Grant date | May 17, 2011 |
| Priority date | — |
| Expiry date | Jul 3, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides mutant Mycobacterium strains harboring a modified tyrosine phosphatase gene (mptpA or mptpB) wherein the mutant Mycobacterium strain is incapable of expressing the active tyrosine phosphatase. The invention provides a method for developing the said mutant strain from either Mycobacterium tuberculosis or Mycobacterium bovis. The mptpA or mptpB gene may be modified by replacing the internal sequences with an antibiotic resistance marker gene, which disrupts the expression of the active gene. The invention further provides a recombinant vector comprising the modified mptpA or mptpB which may be used to develop the mutant strains of mycobacteria. The invention provides a method to assess the role of tyrosine phosphatases MptpA and MptpB in the virulence and pathogenesis of Mycobacterium which can be used as potential targets for developing anti-tubercular drug.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.