Method and device for determining the fluorescence of a sample and use thereof
US7943910B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 15, 2006 |
| Grant date | May 17, 2011 |
| Priority date | — |
| Expiry date | Aug 23, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/645
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In a method and a device for determining the fluorescence of a sample, wherein the sample (40) is irradiated by light (25) of a wavelength which is suitable to excite fluorescent light (27, 28) in the sample and the fluorescent light (28) emitted by the sample is received in a receiver (24) and converted into a measurement signal, wherein reference light (32) in particular for compensating for ambient influences is additionally fed to the receiver (24) and likewise converted into a reference measurement signal, provision is made for the optical path of the excitation light (25) entering the sample (40) and fluorescent light (27, 28) leaving the sample (40) to be separated from the optical path (32) of the reference light having the same wavelength as the excitation light between light sources (21, 31) and receiver (24), by means of which a more precise evaluation of the fluorescent light emitted by a sample (40) can be achieved in addition to a simplified design complexity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.