Methods and organisms for the growth-coupled production of 1,4-butanediol
US7947483B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 10, 2007 |
| Grant date | May 24, 2011 |
| Priority date | — |
| Expiry date | Dec 30, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.