Protein detection and quantitation using hydroxyquinolone dyes

US7947506B2 · kind B2 · utility

Assignee

• Pierce Biotechnology, Inc. · US

Inventors

• Brian David Wolf · Machesney Park, US
• Surbhi Desai · Rockford, US
• Peter T. Czerney · Weimar, DE
• Frank G. Lehmann · Jena, DE
• Bernd G. Schweder · Jena, DE
• Matthias S. Wenzel · Jena, DE

Key dates

• Filing date: Jun 2, 2006
• Grant date: May 24, 2011
• Expiry date: Jul 28, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6839
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A hydroxyquinolone compound formulated as a fluorescent dye for protein detection, assay, quantitation, etc. The hydroxyquinolone compound may be modified, for example, by adding or removing sulfate groups, changing hydrocarbon chain lengths, etc. to result in more desirable properties such as enhanced binding to basic proteins, enhanced solubility, etc. The dye has enhanced sensitivity over commercially available protein stains, and may be used to stain proteins in solution, proteins separated on gels, proteins transferred to solid supports, etc. Methods of using the dyes are also disclosed.