Two-stage nucleic acid amplification using an amplification oligomer
US7951539B2 · kind B2 · utility
11Cited by
6References
21Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 7, 2009 |
| Grant date | May 31, 2011 |
| Priority date | — |
| Expiry date | Oct 7, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods, compositions and systems to detect a nucleic acid of interest in a two-stage amplification. The two-stage amplification begins with a first non-enzymatic accumulation of an amplification oligomer that is the target substrate for a second nucleic acid amplification or assay. Two or more amplification oligomers can be used to allow multiplexed amplifications of two or more nucleic acids of interest with deconvolution based on unique detection signals or unique signal locations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.