Construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof
US7951572B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 27, 2007 |
| Grant date | May 31, 2011 |
| Priority date | — |
| Expiry date | May 21, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2610/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is a gold nanoparticle (AuNP)-based peptide chip prepared by forming a monolayer of AuNPs onto a self-assembled monolayer constructed on a solid support, and then immobilizing a peptide on the AuNPs. The AuNPs can effectively amplify the mass signal of the peptide, thus making it possible to measure the mass change of the peptide in a simple and accurate manner. Also, when secondary ion mass spectrometric analysis (spectrum or imaging) is performed on the AuNP-based peptide chip, the activities of enzymes and related inhibitors can be effectively quantified. The disclosed invention enables various enzyme activities to be analyzed rapidly and accurately, and thus can provide an important method for disease diagnosis and new drug development through the elucidation of signaling and interaction mechanisms.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.