Automated, programmable, high throughput, multiplexed assay system for cellular and biological assays

US7951580B2 · kind B2 · utility

Assignee

• The Regents of the University of California · US

Inventors

• Guann-Pyng Li · Irvine, US
• Mark Bachman · Irvine, US
• Nancy Allbritton · Irvine, US
• Chris Sims · Irvine, US
• Cynthia Jensen-McMullin · Laguna Hills, US

Key dates

• Filing date: Apr 21, 2005
• Grant date: May 31, 2011
• Expiry date: May 11, 2026

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N15/149
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Systems and methods are providing for performing high-throughput, programmable, multiplexed assays of biological, chemical or biochemical systems. Preferably, a micro-pallet includes a small flat surface designed for single adherent cells to plate, a cell plating region designed to protect the cells, and shaping designed to enable or improve flow-through operation. The micro-pallet is preferably patterned in a readily identifiable manner and sized to accommodate a single cell to which it is comparable in size. Each cell thus has its own mobile surface. The cell can be transported from place to place and be directed into a system similar to a flow cytometer. Since, since the surface itself may be tagged (e.g., a bar code), multiple cells of different origin and history may be placed into the same experiment allowing multiplexed experiments to be performed.