Cytochrome p450 monooxygenases and their use for oxidizing organic compounds

US7960155B1 · kind B1 · utility

Assignee

• BASF SE

Inventors

• Bernhard Hauer · Neheim, DE
• Juergen Pleiss · Asperg, DE
• Ulrich Schwaneberg · Waiblingen, DE
• Jutta Schmitt · Stuttgart, DE
• Markus Fischer · Ludwigsburg, DE
• Rolf Schmid · Gelterkinden, CH
• Qing-shan Li · Kyoto, JP
• Sabine Lutz-Wahl · Kirchheim unter Teck, DE
• Daniel Appel · Kaufbeuren, DE

Key dates

• Filing date: Jul 27, 2000
• Grant date: Jun 14, 2011
• Expiry date: May 7, 2021

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/90245
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to novel cytochrome P450 monooxygenases comprising a modified substrate specificity, to nucleotide sequences which code therefor, to expression constructs and vectors containing these sequences, and to microorganisms transformed therewith. The invention also relates to methods for microbiologically oxidizing different organic substrates, such as methods for producing indigo and indirubin.