Methods for culture and production of single cell populations of human embryonic stem cells
US7964402B2 · kind B2 · utility
46Cited by
4References
22Claims
0Family size
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Key dates
| Filing date | May 24, 2007 |
| Grant date | Jun 21, 2011 |
| Priority date | — |
| Expiry date | Aug 18, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2509/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
We used Accutase™, a commercially available cell detachment solution, for single cell propagation of pluripotent hESCs. Unlike trypsin dissociation, Accutase treatment does not significantly affect the plating efficiency of hESC dissociation into single cells. Cultures dissociated with Accutase to single cells at each passage maintain a higher proportion of pluripotent cells as compared to collagenase-passaged hESCs. Accutase-treated hESCs can be grown to a high density as monolayers, and yet retain their pluripotency.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.